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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
12个月
- 英文名:
DIHYDRORHODAMINE 123 10055
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes (1). Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) (2,3) and peroxynitrite (4,5). Also see dihydrorhodamine 123 dihydrochloride (10056), a more stable and water soluble form of dihydrorhodamine 123.
- White solid soluble in DMSO
- Store at -20°C and protect from air and light, especially when in solution
- C20H18N2O3
- MW: 346
- [109244-58-8]
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文献和实验Fluorogenic Analysis of H2O2 in Biological Materials
-dichlorodihydrofluorescein (H 2 DCF, also called dichlorofluorescin) or dihydrorhodamine 123 (H 2 RD123) (Fig. 1 ). These dyes were originally used to measure peroxidase activities ( 3 ) but the assays were easily modified to allow peroxide determination ( 2 , 4 –
Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis
dihydrorhodamine, which reacts with ROS such as singlet oxygen and hydrogen peroxide to form fluorescent rhodamine-123. Unlike confocal microscopic methods, two-photon excitation provides depth penetration through the epidermis and dermis with little photodamage
Measurement of Oxidative Burst in Neutrophils
on changes in the fluorescence properties of dihydrorhodamine 123, which can be assessed by flow cytometry.













