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文献和实验CHAPS Insoluble Floating Fraction Preparation
7.5, 50 mM NaCl, 2 mM EDTA). They were finally resuspended in TEBP (TEB plus various protease inhibitors: 1x PIC I and 1x PIC II) to 2 x 108 /ml. CHAPS powder was added at 20 mg/ml. After gentle mixing, the lysed cells were kept on ice for 3-5
into the top tank where the solution is ready for use. 10% formal-saline solution can be dispensed from the tap with the red knob on the cut-up bench. NB: See protocol on formaldehyde safety and COSHH form for formaldehyde for the hazards associated
10%过硫酸铵溶液 【配制方法】 把1g过硫酸铵溶解于终量为10ml的水溶液中，该溶液可在4℃保存数周。 (责任编辑：大汉昆仑王)
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