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文献和实验Preparation of Microinjection Buffer
Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl
10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
cells/ml in complete RPMI medium (A1) TTE solution: TE buffer pH 7.4 (A1) with 0.2% Triton X-100 (store at 4°C) NaCl 5M, ice cold Isopropanol, ice cold Ethanol at 70%, ice cold TE buffer pH 7.4 (A1) Loading buffer 10x (A1) TBE buffer
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