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文献和实验Agar Plates for Selection of Clones in Bacteria
Cloning of PCR products Stocks: LB Agar: Luria Broth after Lennox: per Liter Tryptone 10 g Yeast Extract 5 g
Preparation of Antibiotic Stock Solutions and Agar Plates
, which sometimes has pH=9-10 adjusting the pH can be important! Preferred storage temperature is -70℃! KanamycineI also observed impaired activity of Kanamycin after several months at -20℃! 50 mg/ml is suitable concentration for stocks. Add 200 µl to 100 ml LB-Agar/LB
Preparing Antibiotics Stock Solution and Ampicillin Agar Plates
for stocks. Add 200 µl to 100 ml LB-Agar/LB-Medium (100 µg/ml). 200 µg/ml for Ampicillin works also fine. Preparation of Agar Plates Agar Recipes: 10 g of NaCl 10 g of Tryptone 5 g of Yeast Extract 20 g of Agar
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