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文献和实验. coli strain . Solutions: 10x Ligation Buffer: 0.5 M Tris-HCl pH 7.8, 50 mM MgCl2 , 0.1 M b-mercaptoethanol, 50 mM ATP, 5 mg/ml BSA
4.2 ml incubate for 2-3 h (or ovn) at 14℃. proceed with the transformation of the appropriate E. coli strain . Solutions: 10x Ligation Buffer:0.5 M Tris-HCl pH 7.8, 50 mM MgCl2 , 0.1 M b
Standard hybridization technique
on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984). You will need: 6 x SSC (0.9M NaCl,90mM sodium citrate,pH 7) 10% SDS 50mg/ml heparin
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