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- CAS号:
7732-18-5
- 规格:
100ML
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文献和实验Access RT-PCR System (Promega)
and purity of the mRNA used as the template. Use procedures for creating and maintaining an RNase-free environment, use sterile tubes, pipette tips, gloves and diethyl pyrocarbonate (DEPC)-treated water. When isolating RNA from samples high in ribonuclease
E-Z 96 X-press Plasmid Spin Protocol
). 7. Add 100-150μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. 8. Centrifuge at 3000 x g for 5 minutes to elute plasmid DNA.
Fungal Midi DNA Kit Protocol for Fresh/Frozen Specimens
1. Centrifuge capable of at least 8,000 x g 2. Nuclease-free 15 mL or 20 mL high speed Carbonate Thick wall centrifuge tubes 3. Waterbath equilibrated to 65°C 实验步骤 1. Collect ground Fungal tissue (start with 500 mg) in a 15 mL
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