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文献和实验1.00μm 2318.40 OV-11通用型毛细管色谱柱 30m×0.25mm×0.33μm 1849.20 OV-11通用型毛细管色谱柱 30m×0.25mm×0.50μm 1918.20 OV-11通用型毛细管色谱柱 30m×0.25mm×1.00μm 2732.40 OV-11通用型毛细管色谱柱 50m×0.25mm×0.33μm 3008.40 OV-11通用型毛细管色谱柱 50m×0.25mm×0.50μm 3146.40 OV-11通用型毛细管色谱柱 15m×0.32mm×0.33μm
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
. Add Electrophoresis DNA Sample Buffer. Mix by vortexing 10 min to dissolve the DNA completely. 11. Pour a 1.5 mm 8% native DNA preparative gel consisting of the following components (see Protocol on Preparation of Non-denaturing Preparative
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