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文献和实验Phycoerythrin conjugation protocol
DMSO Dimethyl sulphoxide, anhydrous SPDP 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (Sigma) SMCC Succinimidyl trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate
Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds
the microtubule polarity by measuring the differences in the dynamic properties of the ends alone. In this chapter, we describe a method for labeling tubulin protein with N -hydroxysuccinimide ester fluorescent dyes, enabling the formation of dual-color polarity
-hydroxysuccinimide ester (PSE), which yields the formation of the fluorescence intramolecular excimer. This chapter provides a detailed description of these two principal derivatization methods used for HPLC determination of histamine in the microdialysis
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