Genopure Plasmid Midi Kit

Fastfilter Plasmid Midi Kit Spin Protocol

with Lysate Clearance Filter Syringe. Note: Step 10 to 16 should be performed in swinging-bucket rotor for maximal plasmid DNA yields. All of centrifugation steps must be carried out at room temperature. Purify Plasmid DNA with HiBindTM DNA Midi

QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒

      ♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒

PLASMID MIDI-PREP FROM BACTERIA

of culture should produce ~0.5-1.0 mg of plasmid DNA from a high copy plasmid) 23. Optional: determine the amount that is barely visible on a THIN EtBr-agarose gel Note: EtBr has a visibility limit of about 100-200 ng