Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases.
Application
cOmplete™, EDTA-free Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay (RIPA) lysis buffer for resuspending Chinese hamster ovary (CHO) cells. It has also been used as a component of resuspension buffer for HCT116 colorectal cancer cells.
cOmplete™, EDTA-free Protease Inhibitor Cocktail has been used as as a component of • immunoprecipitation lysis buffer (IPLS) for lysis of human embryonic kidney cells 293 (HEK293T cells) for glutathione co-precipitation • radioimmunoprecipitation assay (RIPA) buffer for lysis of cells for western blotting • buffer A for the lysis of bacterial cells