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Recombinant Human Mesencephali

c Astrocyte-Derived Neurotrophic Factor
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  • ￥1080 - 45885
  • PROSPEC
  • 以色列
  • cyt-141
  • 2025年12月26日
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    上海经科化学科技有限公司
    16 年钻石会员

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    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      MANF

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      5ug/20ug/1mg

    规格：5ug产品价格：￥1080.0
    规格：20ug产品价格：￥2415.0
    规格：1mg产品价格：￥45885.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-141

    SYNONYMS

    Mesencephalic astrocyte-derived neurotrophic factor, Protein ARMET, ARP, arginine-rich mutated in early stage tumors, Arginine-rich protein.

    INTRODUCTION

    MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

    DESCRIPTION

    MANF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18.1 kDa.
    The MANF is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    Filtered (0.2µm) and lyophilized from a concentrated (1mg/ml) solution in 1×PBS, pH7.4.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized MANF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized MANF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MANF should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL.

    BIOLOGICAL ACTIVITY

    The ED50 was determined by its ability to stimulate the proliferation of rat C6 cells is typically 15-25 µg/ml.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    该产品被引用文献

    MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

    相关实验

    • Production of Recombinant Neurotrophic Factors

      factor (NGF), and its related protein family members, brainderived neurotrophic factor (BDNF or NT-2), and neurotrophin3 (NT.-3). The potential of producing mg and larger quantities of these agents will expedite our understanding of their functions

    • Compartmented Chambers for Studying Neurotrophic Factor Action

      Neurotrophic factors released by target tissues maintain the survival and differentiation of innervating neurons. The manner by which these target-derived neurotrophic proteins communicate with innervating neurons has been actively pursued

    • Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)

      The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true

    查看更多相关实验 >

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    文献支持
    Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor
    ￥1080 - 45885