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- PROSPEC
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- cyt-205
- 2025年12月24日
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- 详细信息
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- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
IFNA2B
- 库存：
部分小规格有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
20ug/100ug/1mg
| 规格： | 20ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥14500.0 |
CATALOGUE NUMBER
CYT-205
SYNONYMS
IFN alpha 2b, IFNA, INFA2, MGC125764, MGC125765.
INTRODUCTION
IFN-alpha is produced by macrophages and has antiviral activities. IFN stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
DESCRIPTION
IFN-a 2b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine.
The IFN-alpha 2b gene was obtained from human leukocytes.
The IFN-a 2b is purified by proprietary chromatographic techniques.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
SOLUBILITY
It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized IFN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PURITY
(b) Analysis by SDS-PAGE.
AMINO ACID SEQUENCE
MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
BIOLOGICAL ACTIVITY
PROTEIN CONTENT
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN alpha 2b as a Reference Standard.
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文献和实验Title:IFN-β induces apoptosis in human SH-SY5Y neuroblastoma cells through activation of JAK–STAT signaling and down-regulation of PI3K/Akt pathway.
Publication:Article first published online: 11 NOV 2010 DOI:10.1111/j.1471-4159.2010.07046.x © 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry.
motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
【求助】哪里能买到含有人干扰素alpha-2b cDNA序列的质粒pBV889
baiguo861029 各位前辈好 本人是个新手，听很多师兄师姐说丁香园很好，这儿的人都很热情帮助他人。我现在研究生在读，因为课题需要，急需购买这个质粒pBV889，试了好多方法都没找到。这个质粒含有人干扰素alpha-2b编码序列，即cDNA序列。非常希望知道的前辈给予我帮助，非常感谢！！ woxingwosu 用google搜索了一下，发现很多文章都有，国内发表的也很多。其实你或者你老板可以写信去要一下就方便多了，还不用
Many cytokines alter the half-lives and stabilities of mRNAs for other cytokines (1 ) and enzymes that may be related to disease progression. For example, IL-1β stabilizes the mRNA for human interstitial collagenase-1 in normal fibroblasts
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