Recombinant Porcine Leptin产品图
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Recombinant Porcine Leptin

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  • ¥1600 - 30429
  • PROSPEC
  • 以色列
  • cyt-503
  • 2025年12月26日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件:

      for future use below -18°C

    • 保质期:

      See instructions

    • 英文名:

      pLeptin

    • 库存:

      部分小规格有备货

    • 供应商:

      上海经科化学科技有限公司

    • CAS号:

      无

    • 规格:

      20ug/100ug/1mg

    规格:20ug产品价格:¥1600.0
    规格:100ug产品价格:¥5000.0
    规格:1mg产品价格:¥30429.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-503

    SYNONYMS

    OB Protein, Obesity Protein, OBS, Obesity factor.

    INTRODUCTION

    A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

    DESCRIPTION

    Leptin Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and additional Ala at N-terminus, having a molecular mass of 16kDa.
    The Leptin is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized recombinant porcine leptin in sterile 0.4% NaHCO3 adjusted to ph 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 98.0% as determined by:
    (a) Gel filtration analysis.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Trp.

    BIOLOGICAL ACTIVITY

    Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC,using calibrated solution of Leptin Porcine as a Reference Standard.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Leptin Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and additional Ala at N-terminus, having a molecular mass of 16kDa.
    The Leptin is purified by proprietary chromatographic techniques.

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    文献支持
    Recombinant Porcine Leptin
    ¥1600 - 30429