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Recombinant Human Neurotrophin

-3
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  • ￥1080 - 79695
  • PROSPEC
  • 以色列
  • cyt-257
  • 2025年12月27日
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    上海经科化学科技有限公司
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    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      NT3

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      2ug/10ug/1mg

    规格：2ug产品价格：￥1080.0
    规格：10ug产品价格：￥2415.0
    规格：1mg产品价格：￥79695.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-257

    SYNONYMS

    Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3.

    INTRODUCTION

    NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

    DESCRIPTION

    Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.
    The NT-3 is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    Lyophilized from 0.02% TFA.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 97.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by the dose-dependent proliferation of C6 cells and is 3.6-5.4?g/ml.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    SAFETY DATA SHEET

    SDS

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    图标文献和实验
    该产品被引用文献

    1.Title:Generation of Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant[1]^†[2]^‡.
    Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press.

    4.Title: Orphan nuclear receptor Nurr1 induces neuron differentiation from embryonic cortical precursor cells via an extrinsic paracrine mechanism
    Publication:  FEBS letters 583.9 (2009): 1505-1510

    相关实验

    • Visualization and Quantitation of Neurotrophin mRNAs

      ��k et al. 1991). Recently, a mammalian homologue of NT-4 has been molecularly cloned from rat and human (Ip et al., 1992). The same sequence was also recently isolated by Berkemeier et al. (1991) who named it neurotrophin-5 (NT-5

    • Construction of Recombinant Human Cytomegalovirus

      The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral

    • Delivery of Recombinant Adenoviruses to Human Saphenous Vein

      The human saphenous vein is the most commonly used conduit for coronary artery bypass grafting owing to its ready availability, ease of harvesting, and favorable surgical handling (1 ). However, it suffers from a progressive decline

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    文献支持
    Recombinant Human Neurotrophin-3
    ￥1080 - 79695