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Recombinant Porcine Interleuki

n-1 alpha
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  • ￥1080 - 21735
  • PROSPEC
  • 以色列
  • cyt-396
  • 2025年12月24日
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    上海经科化学科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      pIL1A

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      2ug/10ug/100ug

    规格：2ug产品价格：￥1080.0
    规格：10ug产品价格：￥2415.0
    规格：100ug产品价格：￥21735.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-396

    SYNONYMS

    Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha, IL1, IL-1A, IL1F1.

    INTRODUCTION

    Interleukin-1 alpha is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. Circulating levels of are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1 alpha possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.

    DESCRIPTION

    Interleukin-1A Porice Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton.
    The IL-1A is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by the dose-dependant stimulation of D10S cells is < 0.03 ng/ml.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Interleukin-1A Porice Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton.
    The IL-1A is purified by proprietary chromatographic techniques.

    相关实验

    • Recombinant Cell Lines Stably Expressing Functional Ion Channels

      acetylcholine receptor alpha7 (nAChRα7) in a host cell, we show a way to construct and use such a stable cell-based expression system for electrophysiological assays.

    • 核酸标记技术检测法

      and helical cytokines .Immunol Today,1990;11(10):350 5.Cannon J G,et al.Circulating interleukin-1 and tumor necrosis of tumors.PNAS,1975;72:3666 7.Chan SH,et al.Induction of interferon γ production by natural killer cell stimulatory factor

    • 核酸标记技术检测法

      and helical cytokines .Immunol Today,1990;11(10):350 5.Cannon J G,et al.Circulating interleukin-1 and tumor necrosis of tumors.PNAS,1975;72:3666 7.Chan SH,et al.Induction of interferon γ production by natural killer cell stimulatory factor

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    Recombinant Porcine Interleukin-1 alpha
    ￥1080 - 21735