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文献和实验These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
Single Primer ("Semi-Random") P
1 (TP698) 214 bp prior to gene facing upstream. GTCACAGGTATTTATTCGGCGCA Po: CKO3 (TP45) 154 bp past gene facing downstream. AGGGCAGGGTCGTTAAATAGC Pn: CMR3 (TP700) 223 bp past gene facing downstream. AGTGTGACCGTGTGCTTCTCAA
Preparation of Mitochondria from Rat Liver
Preparation of Mitochondria from Rat Liver Rat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawley albino male rats for our studies. Female rats can be used, however for serious studies
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