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- 2025年07月11日
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文献和实验Staining Procedure for Flow Cytometric Detection of Human Cyclins
No. 3 above. II. Staining 1. Just prior to staining, remove ethanol by centrifugation at 200 x g for 10 min. Aspirate and wash once by resuspending pellet in 30-40 m l of Wash buffer. Centrifuge at 200 x g for 10 min
Long-PCR Reagents and Guidelines
0.02 U Vent 0.2 mM each dNTP 20-40 pmoles each primer (400-800 nM) 1.1-1.2 mM Mg(OAc)2 105 -107 template molecules For PCR with moderate-complexity templates (e.g., bacterial genomic DNA) 50ml reaction
Isolate It All：siRNA • miRNA • Total RNA • Native Protein
) were also enriched ~10 fold (Figure 3B). Figure 3. Small RNA Enrichment from Cultured Cells. Total RNA or fractions depleted or enriched in small RNA were isolated in triplicate from 1 x 106 HeLa
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