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文献和实验Identification of Protein Interactions by Far Western Analysis
. Grulich‐Henn, J., Spiess, S., Heinrich, U., Schonberg, D., and Bettendorf, M. 1998. Ligand blot analysis of insulin‐like growth factor‐binding proteins using biotinylated insulin‐like growth factor‐I. Horm
A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
-like growth factor-1 (IGF-1) compared with nonstimulated cells. Further addition of a fourfold molar ratio of IGF binding protein-3 completely abolished this effect. This method can be used to measure proteoglycan synthesis by chondrocytes from many species
, Hedgehog, and insulin pathways, as well as the activationof nuclear factor (NF)-kB-mediated gene transcription. These signaling pathways regulate gene transcription,cell cycle, apoptosis, inflammation, glucose metabolism, stem-cell renewal
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