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文献和实验Fluorescence In Situ Hybridization on Early Porcine Embryos
, which are necessary to remove the lipid, have the effect of flattening the nuclear structure. The work in this chapter has focussed on the pig; however, such methods could be applied to bovine, ovine, and canine embryos, all of which are rich in lipid.
Group 2a of the Coronaviridae family contains human and animal pathogens that include mouse hepatitis virus, rat coronavirus, human respiratory coronaviruses OC43 and the recently identified HKU1 strain, a newly recognized canine respiratory
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
, then sink in 30% sucrose in PBS + Mg2+at 4O C. Fixation times will vary with the size and nature of the tissue. For embryonic chick brains of E10 or older and for postnatal rat or mouse brains we incubate up to 8 hours in fixative. Perfusion
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