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文献和实验Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b
Introduction to the EMSA (Gel Shift) Technique
are used to accommodate assembly of multiprotein complexes. If the sequence is larger (100-500 bp), the DNA source is usually a restriction fragment or PCR product obtained from a plasmid containing the cloned target sequence. Protein:DNA complexes formed
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