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货号：610176
名称：PKC IOTA MAB 150UG 23
品牌：BD biosciences
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文献和实验T-Cell Activation Using mAb to CD3
of specific protein tyrosine kinases (PTKs), breakdown of phosphatidylinositol 4,5-biphosphate (PIP2), activation of protein kinase C (PKC) and elevation of intracellular calcium ion concentration. These early events are transmitted to the nucleus and result
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Intracellular Immunofluorescent Staining for Flow Cytometry
type and the experimental conditions. For example, to stimulate T cells to produce IFN-γ, TNF-α, IL-2, and IL-4, a combination of PMA (a phorbol ester / PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies can be used. To induce IL
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