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货号:558639
名称:NHP T/B/NK CELL CKTL 50TST
品牌:BD biosciences
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文献和实验(for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend in 40 ml R10 media. Spin again and resuspend in 50 x the original cell
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
perpendicular to the plane of contact of the two cells. The pulse parameters varry depending on the electrodes and pulse generator. We use Cell-fusion instrument, CF-150B available from BLS Ltd., Hungary with following parameters (for non-electrolyte fusion
effector cell concentration to 4 x 106 cells / ml (for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend
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