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货号：558495
名称：RAT T/B/NK CELL CKTL 50TST
品牌：BD biosciences
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文献和实验Fusion of Mouse, Rat, or Hamster Cell
-well plate containing 5 ml/well sterile PBS. Transfer the spleen to a Petri dish containing 50 ml sterile PBS. Prepare a single cell suspension by teasing the tissue with sterile forceps
Preparation of Rat Liver Cell Cytosol
(Beckman # 344058) Preparation of Rat Liver Cytosol Chill the superspeed centrifuge, ultracentrifuge, SS-34 and 50.2Ti rotor to 4oC. In the cold room, rinse the liver well in PBS, then rinse in freshly prepared Homogenization
(for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend in 40 ml R10 media. Spin again and resuspend in 50 x the original cell
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