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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACK-1.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
H, M, R
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACK-1.
- 目录编号:
251595
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
ACK-1
- 抗体英文名:
ACK-1 Antibody
- 抗体名:
ACK-1 Antibody
- 规格:
0.1 mg
ACK-1 Antibody产品详细介绍:
| 产品名称: | ACK-1 Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 251595 |
| 规格: | 0.1 mg |
| 产品描述: | ACK-1 (Activated CDC42 kinase 1) is a tyrosine kinase which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. ACK-1 binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. ACK-1 also participates in clathrin-mediated endocytosis. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACK-1. |
| 适用物种: | H, M, R |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500 |
| 别名: | ACK-1; Activated CDC42 kinase 1; Tyrosine kinase non-receptor protein 2; TNK2; ACK1 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验对检测结果进行定性定量分析。 检测分析结果在生物、医学上的意义进行分析和评价。 三、外周血液标本的制备 a)常规血液样本制备 采集外周血,用肝素抗凝;采集后室温(25℃)保存,6 h 内处理; 红细胞裂解液(ACK buffer)取出后,恢复至室温待用; 流式管内加入 200 ul 外周血后,加入 3 mL 的 ACK buffer,室温孵育 3~5 min; 加入 10 mL 细胞染色buffer(或含 1%BSA 的 PBS)终止红细胞裂解; 300 g 离心细胞悬液 5 分钟,弃掉上清
FACS Analysis Using Peripheral Blood Cells
) and mix immediately to prevent clotting. Keep tubes on ice. Remove RBCs from samples. This can be accomplished by several means. At The Jackson Laboratory, RBCs are lysed using either Gey's solution or a buffered ammonium chloride (ACK) solution
. At The Jackson Laboratory, RBCs are lysed using either Gey's solution or a buffered ammonium chloride (ACK) solution. (Alternatively, Becton-Dickinson sells a product called "FACS lysis buffer" that is used after the staining protocol to lyse RBCs and fix
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