重组蛋白G

Recombinant Protein G，重组蛋白G
Catalog Number: PL030361P
Quantity size : 1mg; 10mg
Resources: Escherichia coli (E. coli)
Purity: ≥95% by SDS-PAGE analysisBackground: Protein G is a bacterial cell wall protein expressing at the cell surface of some group C and group G Streptococcal strains that binds the Fc region of immunoglobulin G (IgG) with high affinity. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to IgGs from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G shows a broader range of binding to IgG subclasses than does staphylococcal protein A. Some of the most important application areas for protein G are the isolation and purification or the removal of IgG from serum, the purification of monoclonal antibodies, and the isolation of immune complexes. Protein G conjugates is commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites, tissue culture supernatants and other biological fluids. In addition to the Fc receptor, intact protein G has membrane spanning regions as well as specific binding sites for albumin and for the Fab region of immunoglobins. The albumin and cell surface binding domains have been eliminated from recombinant protein G to ensure the maximum specific IgG binding capacity.
Storage: Store at –20 ºC for three years. Avoid repeated freeze/thaw cycles. The lyophilized protein is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. It is recommended that the product is reconstituted with sterile water into a final concentration of 10 mg/ml freshly.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

品 名：重组蛋白G（Recombinant Protein G）
货 号：PL030361P
规 格：1 mg，10 mg，100 mg，500 mg
产品形式：冷冻干粉；冻干前缓冲体系为5 mM PB（pH 7.4）
分 子 量：28 kDa
等 电 点：4.79
A280nm(1 mg/mL)：1.350
来 源：大肠杆菌
纯 度：≥95%
保存条件：-20℃保存
有 效 期：3年
相关介绍：
链球菌G蛋白（Streptococcus Protein G 简称SPG）是G、C型链球菌细胞壁中的一种蛋白质，能特异性地同免疫球蛋白IgG分子的Fc段结合而不影响其Fab段与抗原分子结合的能力。它不仅能与人、小鼠、大鼠IgG的全部亚类相结合，还可与豚鼠、兔、山羊、牛、绵羊和马等的IgG相结合，明显优于只能结合人、小鼠IgG的金黄色葡萄球菌蛋白A（Staphylococcus Protein A，简称SPA）。
SPG的用途极为广泛，可用于从血清中分离并纯化或去除IgG、单克隆抗体的纯化和抗原抗体复合物的分离等。SPG与琼脂糖凝胶结合后可制备用于抗体纯化的亲和填料，从血清、腹水、细胞培养上清等中分离和纯化不同亚型的IgG。
天然SPG除含有IgG结合域之外，同时还具有白蛋白和细胞表面结合域。重组SPG去除了白蛋白和细胞表面结合域，以减少非特异性结合，它比天然SPG和SPA具有更大的亲和力。