Merck层析板，1.05747.0001 silica g

Silica Clean-up of DNA

for downstream digests oheat 5 minutes at 60 degrees ospin 2 min @ full speed in microcentrifuge oremove DNA-containing supernatant to clean tube Notes & Misc: •Conveinient PCR clean up before digestion: 100μl PCR reaction cleaned up with 50μl silica

Silica Clean-up of DNA

resuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer for downstream digests heat 5 minutes at 60 degrees spin 2 min @ full speed in microcentrifuge remove DNA -containing supernatant to clean tube Notes & Misc

Agarose Gel Electrophoresis of DNA

gel. 2) Cast the gel with the comb in place. 3) Add 6X gel loading buffer to sample and load the samples into wells. 4) Run the gel submerged in 1X TAE or TBE (30-60 min. at 100-150V) 5) Stain the gel with ethidium bromide solution (10 µl