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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
—20℃,避光,12个月
- 库存:
100
- 供应商:
上海远慕生物
- 规格:
5ml
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文献和实验MITOMYCIN C TREATMENT OF PMEFs
/ml = 200 ug/ ml (= 20 x). Filter sterilize. Use at 1/20 to give a working concentration of 10 ug/ml. Store at 4� wrapped in foil as mitomycin C is light sensitive. Don't use after 6 weeks old. NB. Mitomycin C is very cytotoxic, take care
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
蔗糖水溶液的密度 g/mL ( 摘自 P.Honig: "Principles of Sugar Technology" Vol.I, p31 ) 克蔗糖/100克溶液 0℃ 10℃ 15℃ 20℃ 25℃ 30℃ 40℃ 50℃ 60℃ 0 0.99987 0.99973 0.99913 0.99823 0.99707 0.99567 0.99232 0.98813 0.98330 5 1.02033 1.01960 1.01884 1.01785 1.01661
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