- ￥3996
- Abcam
- ab25029
- 2025年07月09日
- BL,FC
- Ms
- Ms
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Mouse monoclonal [10-1.D.2] to CD72.1 (FITC)
- 应用范围：
BL,FC
- 宿主：
Ms
- 适应物种：
Ms
- 保存条件：
4°C/-20°C
- 亚型：
IgG2b
- 规格：
100ug
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文献和实验Polyclonal and Monoclonal Antibodies
, boost again a month later). 3) 7 - 10 days later bleed the mouse and test serum using the assay which will be used for screening. If titre is not high enough, boost again two weeks after previous boost. Serum Preparation:
Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Western Blotting with Monoclonal Ant
Sample Preparation For Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate excess PBS. 3.Add 1ml boiling lysis buffer
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