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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Cd72 (CD72.1 alloantigen) mouse monoclonal antibody, clone 10-1.D.2, FITC
- 抗体英文名:
Cd72 (CD72.1 alloantigen) mouse monoclonal antibody, clone 10-1.D.2, FITC
- 靶点:
Cd72
- 应用范围:
FC
- 宿主:
Mouse
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM08051FC-N
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing.
- 形态:
PBS containing 0.09% Sodium Azide as preservative.Label: FITCState: Liquid purified Ig fraction.Label: Fluorescein Isothiocyanate Isomer 1
- 免疫原:
DBA/2 mouse spleen cells. (Ref.1)
- 规格:
500 ug
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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