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- Clontech
- USA
- 639605
- 2025年08月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
In-Fusion™ Dry-Down PCR Cloning Kit
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
96 rxns
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文献和实验Inverse PCR & Cycle Sequencing
) 2.0ul 1) Incubate O/N at 4°. 2) Ethanol precipitate on ice, 10min. 3) Spin, aspirate, pulse, aspirate. 4) Speed Vac briefly to dry pellet. 5) Resuspend in 150ul TE (~1/150 fly per ul). IV. PCR Set up PCR reactions
Mutagenesis by PCR(adapted from Ito et al , Gene 102, 67-70) This method needs only a single mutagenic primer (+ 3 other primers which are the same for all constructs if used in the same vector) and would be a cheap and reliable method if lots
Cleavage Efficiency Close to the Termini of PCR Fragments
When designing PCR experiments in which the synthesized DNA fragment is to be subsequently digested with a RE, it is very important to determine how many extra nucleotides should be added to the 5’-end of the PCR primer
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