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文献和实验低RNases的活性(假设有痕量的RNase残留) 小提示:任何事务有利便有弊,LiCl 沉淀与乙醇沉淀相比,它的沉淀效率稍低。数据显示2.5M的LiCl 的平均沉淀率是74%,而乙醇则是85%。通俗的说乙醇沉淀可以得到更多的RNA。
RNase Protection Assay (Bowtell Lab)
0.5ul 200mM DTT 0.5ul appropriate RNA polymerase Incubate for 90-120min at 41�. 5. Add 0.5ul RNAsin and 0.5ul RNAse free DNAse (5mg/ml). Incubate for 15min at 37�. 6. Add 10ug tRNA and 100ul DDW. Phenol/CHCl3 extract and precipitate with 50ul 5M NH
Purification of Endotoxin Free mAbs
that are not guaranteed endotoxin free you should soak them in 0.5M NaOH and then rinse thoroughly with endotoxin free water. Likewise, assume any source of water or buffers are contaminated unless you have checked them using the standard LAL assay or similar
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