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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 库存:
99
- 供应商:
联硕生物
- 规格:
100ml
保存温度:2-8℃
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文献和实验Ribonuclease A (RNase A), DNase-free
guanidinium thiocyanate plus 0.1 M 2mercaptoethanol and heavy metal ions.Inactivated by phenol/chloroform extraction.Note The working concentration for RNase A is 1-100 µg/ml depending on the application. The enzyme is active under a wide range of reaction
LiCl RNA Preparation (Ambros Lab)
rpm, about 5-10 minutes, room temperature 8) Extract with phenol:chloroform 3x or more until interface is almost completely clear 9) Precipitate by adding LiCL to 0.2 M and 3 volumes of 100% ETOH and put at -20oC for 30 minutes
Oocyte Extracts for the Study of Meiotic M-M Transition
In meiotic cell cycles, meiosis I (MI) is followed by meiosis II (Mil) without an intervening S phase, whereas in mitotic cell cycles, an S phase necessarily alternates with an M phase. For the study of mitotic cell cycles, extracts prepared
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