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文献和实验time of 14 days based on their cobblestone morphology. Once EPCs in culture cover ¼ of the 12-well surface area, expand cells and confirm EPC identity with flow cytometry by testing for presence of surface markers CD31 and absence of CD14, CD45
96-well RNA In Situ Hybridization Protocol
-bottom, flexible vinyl no lid 96-well plate (Falcon #3911) Millipore MAVM 096-01 multi-screen filtration system vacuum manifold Millipore MADV N65 10 DV, 0.65um Durapore; 96-well fitration plates
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
. If the cells are being grown up for Southern analysis, use a pre-gelatinized plate containing 200 ul of fresh M15 media per well. Alternatively, if the clones are being screened using PCR, use a 96-well plate
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