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- 2025年12月03日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
PLATE LAMININ 96 WELL RO
- 库存：
10
- 供应商：
上海伟进生物科技有限公司
- 规格：
5
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文献和实验Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
in trypsin for 5 min and dissociate mechanically. 21. Centrifuge the cells at 3000 rpm for 5 min and resuspend in NEP complete medium. Plate cells individually in 96-well, low cell binding plates or bulk passage multiple spheres
Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) 3) Laminin-1 10-12 μg/ml or FN 20 μg/ml 4) 96-well-plate 5) Crystal violet (5mg/ml in 2%
Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) 3) Laminin-1 10-12 μg/ml or FN 20 μg/ml 4) 96-well-plate 5) Crystal violet (5mg/ml in 2%
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