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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
PLATE LAMININ 96 WELL RO
- 库存:
10
- 供应商:
上海伟进生物科技有限公司
- 规格:
5
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文献和实验Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) 3) Laminin-1 10-12 μg/ml or FN 20 μg/ml 4) 96-well-plate 5) Crystal violet (5mg/ml in 2%
to wells of a 96 well tissue culture plate (Costar #3595), cover, and place at 4 C overnight. Coat in triplicate or quadruplicate. 3) Invert plate and shake out coating solution. Pull off remaining coating solution from each well with a yellow tip
Gene Transfer Using Targeted Filamentous Bacteriophage
GFP-positive cells, resulting from transduction of primary rat olfactory bulb cell culture by epidermal growth factor (EGF)-targeted phagemid particles, observed 96 h after phage addition. Explanted cells were grown for 8 d on polylysine-laminin-coated
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