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V1a Rat Chem-1 Stable Cell Line
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文献和实验Generation of a Stable Cell Line for Constitutive miRNA Expression
Here, a protocol is described to generate stable cell lines for constitutive expression of single cellular or viral miRNA precursors in absence of infection. The procedure comprises cloning of the precursor sequence, generation of the lentiviral expression vector
Functional Analysis of Natural Killer Cell Receptors in the RNK-16 Rat Leukemic Cell Line
, leading to ambiguities in functional characterizations of their antigens. To study members of the various NK cell receptor families, we have developed an NK cell transfection system using the NK leukemic cell line RNK-16.
Robust Cell Line Development Using Meganucleases
Cell line development for protein production or for the screening of drug targets requires the reproducible and stable expression of transgenes. Such cell lines can be engineered with meganucleases, sequence-specific endonucleases
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