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APOPTAG FLUORESCEIN DETECTION
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文献和实验Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence
DNA probes can be labeled using a fluorescein-labeled nucleotide and subsequently detected with a dioxetane-based chemiluminescence system using an antifluorescein antibody conjugated to alkaline phosphatase (1 ,2 ). The use
Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection
Oligonucleotide synthesizers are now commonplace with the result that oligonucleotides can be obtained readily, obviating the need for complex molecular biological techniques. However, the choice between long probes or oligomers depends
Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence
chemiluminescence avoids the fading of results associated with some colormetric detection procedures, used with nonradioactive labeling and detection systems. Hard copy results showing excellent resolution are produced.
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