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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
T7*TAG ANTIBODY AGAROSE
- 规格：
2ML
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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Antibody to an equal amount of Taq DNA polymerase units. ForTaq DNA polymerase at 5 unit per μl, prepare a 1:1 mixture. Mix well using a Vortex Mixer. 3. Add the following components to a sterile 0.5-ml microcentrifuge tube:
Purification and Characterization of His-Tagged Antibody Fragments
Immobilized metal affinity chromatography (IMAC) has become a widely used method for the affinity purification of recombinant proteins and, in particular, of antibody fragments produced in Escherichia coli . IDA-Sepharose and NTA-agarose
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