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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
RapidStep ECL Reagent
- 规格:
100ML
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文献和实验Western blotting using ECL reagent
diluted 1:8000 in TBS-T + 1.5% gelatin for 1 hour.6. Repeat step # 4 (wash)7. Prepare ECL solution for detection: mix equal volume of ECL reagent 1 and 2, (1:1) with final volume regarding of 0.125 ml/cm2 (for a mini gel- 4 ml of each reagent).8. Remove
temp for 1 hour. (Can be left at 4℃O/N here)►Wash briefly 2X then 3X10 min each with TBS-T►Mix an equal volume of detection reagent 1 with detection reagent 2 (usually 2mLs total is sufficient).►Drain excess TBS-T from membrane. Lay membrane on a piece
with 1 X PBST three times, each for 10 minutes.The Trident Universal Protein Blocking Reagent (animal serum-free) (GTX30963) does not contain any animal serum, and can be used for all IHC and ICC kits, ELISAs, and WBs.以 ECL 系统检测信号1. Follow the instructions
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