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文献和实验, and differentiation. More than 50 GSK-3 inhibitors representing a wide range of chemical structures have already been identified,and their utility in the treatment of type II diabetes mellitus, Alzheimer’s disease, bipolar diseases, cancer, andother human
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
phosphorylation. This unit describes the use of phospho?motif antibodies to elucidate the kinase(s) responsible for phosphorylating substrate proteins. Curr. Protoc. Mol. Biol. 101:18.20.1?18.20.11. © 2013 by John Wiley & Sons, Inc. Keywords
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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