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文献和实验) and endonuclease-G (endo-G), from the mitochondria into the cytosol through a process that can be blocked by anti-apoptotic multi-domain Bcl-2 family members (Bcl-2, Bcl-xL, Bcl-w, Mcl-1, A1/Bfl1 and Bcl-B). Cytochrome c release is essential
相关专题 细胞凋亡专题 线粒体 凋亡途径是细胞凋亡的主要途径之一,是目前研究凋亡的热点.各种凋亡刺激信号通过BH3(Bcl-2 homology domain 3)-only蛋白引起Bax(Bcl-2-asslciated protein X)蛋白移位到线粒体外膜并多聚化,形成膜通道,刺激线粒体释放细胞色素C(Cyt C) 和Smac(second mitochondrial-derived activator
Denaturing Gradient Gel Electrophoresis (DGGE)
, the denaturants used are heat (a constant temperature of 60 degrees C) and a fixed ratio of formamide (ranging from 0-40%) and urea (ranging from 0-7 M). The position in the gradient where a domain of a DNA fragment melts and thus nearly stops migrating
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