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文献和实验Antibody–drug conjugates (ADCs) combine the selectivity of a monoclonal antibody with the killing potency of a cytotoxic drug. For an antibody to function as a successful component of an ADC, it needs to bind to the target antigen
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
Western Blotting with Monoclonal Ant
, 0.1% Tween 20). Incubate the blot for 30 minutes at 37˚C, 1 hour at room temperature, or overnight at 4˚C. Incubation with Primary and Secondary Antibodies Primary antibody 1.Dilute the antibody in the corresponding blocking buffer. 2.Decant
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