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文献和实验Double-Stranded DNA Site-Directed Mutagenesis
It is now technically possible to create any desired mutation in a given DNA sequence. So-called site-directed mutagenesis allows the introduction of designed mutations into specific locations. This approach is invaluable for studying gene
Labeling of Double-Stranded DNA Probes with Biotin
Probes labeled with biotin have been used for hybridizations to Southern blots and to chromosomes in situ since the early 1980s (1 ). Nowadays, the labeling of double-stranded DNA probes with biotin is made easy by the availability of labeling
Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA
-made primer for second strand synthesis, useful whether this is to be performed by more reverse transcriptase or by E. coli DNA polymerase 1 (pol 1). An idealized picture is shown in Fig. 1 . Before the double-stranded cDNA (ds cDNA) copy can be cloned
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