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文献和实验Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
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