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文献和实验Isolation of Monoclonal Antibody Fragments from Phage Display Libraries
coat protein. Recombinant phage expressing the resulting antibody libraries in fusion with the coat protein are propagated in Escherichia coli . Phage displaying monoclonal antibodies with specificities for target antigens are isolated
Chaperonins in Phage Display of Antibody Fragments
coat protein (protein3) of bacteriophage M13 phage and is, in this way, both anchored in the phage capsid and exposed on the phage surface, linking specificity and genetic information. This permits direct isolation and sequence determination of the gene
Phage Display Technology for Human Monoclonal Antibodies
filamentous phages. Antibodies (Abs) can be displayed successfully on the surface of phage by fusing the coding sequence of the antibody variable (V) regions to the phage minor coat protein pIII. By creating large libraries, antibodies with affinities
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