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文献和实验Neutralizing Bioassay Protocols
describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. In general, the cytokine bioassay protocols are modified to pre-incubate the cytokine of interest with the specific neutralizing antibody prior to addition
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
and can be quantitated by visual scoring or specialized plate-readers. T-Cell Activation Using mAb to CD3 (eBioscience) This protocol is written as a starting point for examining in vitro proliferation of splenic T-cells stimulated via CD
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