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文献和实验PIC Cross-linking and Immune Precipitation
-link samples in the UV oven: energy = 1250 units with 365nm bulbs9.Concentrate beads,discard supernatant10.Resuspend in 20 microliter txn wash buffer + 2 microliter 1 M DTT11.Incubate 10' at RT,mix occasionally12.Concentrate beads,discard supernatant13
Labeling Tubulin and Quantifying Labeling Stoichiometry
and stoichiometry for tubulin labeled with tetramethylrhodamine (TMR) NHS ester: Tubulin concentration = [(A280 - Contribution of dye to A280 ) x Dilution Factor] / Extinction coefficient of tubulin at 280 nm TMR concentration = (A555 x Dilution Factor
ultraviolet light. The only changes that need to be made is the use of quartz cuvettes instead of glass tubes. Glass absorbs UV light and thus is inappropriate for use in a UV spectrophotometer. An instrument capable of using visible light (usually
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