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文献和实验Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
50‐ and 100‐mL flask with reflux condenser Rubber septa (+15.9 mm) Glass‐fritted funnels with medium
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
-through. Then with the collection tube empty, centrifuge the spin cartridge at 10,000 x g for 2 min at room temperature to completely dry the HiBind® matrix. 11. Transfer the column to a clean 1.5 ml micro centrifuge tube (not supplied with kit) and elute the RNA with 30-50
Native Chromatin Preparation and Illumina/Solexa Library Construction
1. On the day of the experiment, obtain a leukapheresis pack from a blood bank. Dilute 109 leukocytes with 1X PBS (1:1). Pipette 15 mL of LSM into a 50-mL conical tube. Carefully layer the lymphocyte suspension over the LSM. A clear boundary
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