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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
Anion‐exchange HPLC device (JASCO) with an anion‐exchange column (DNAPack PA‐100, semi‐prep, 9 × 250–mm, Dionex), flow 5 mL/min PD‐10 sepharose columns
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to the column. With the spin column inside a 2 ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30-60 seconds min at room temperature. Discard flow-through and proceed to step 6. 6. Place column in a new 2 ml collection tube and add 300
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