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文献和实验polypropylene tubes High-speed centrifuge with cooling system and a swing-out bucket rotor for 14-ml polypropylene tubes Dialysis tubing (0.5-mm thick, 10-kDa pore width) (VWR international) Homogenizer, prechilled on ice We use
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
)) then transfer to an Oakridge tube (aka SS34). Add 4.5 ml of K-acetate (7.5M), mix gently, and keep at minus 80°C for 15-30 minutes. 11. Thaw completely (avoid mixing) and spin at 3,000xg RCFMax (5,000 rpm Beckman JA20 rotor; 4,500 rpm in Sorvall HB
Chromatin Assembly Using Drosophila Systems
and appropriate tubes 12,000 to 15,000 and 3,500 MWCO dialysis tubing FPLC apparatus
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