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文献和实验Dialysis (using dialysis tubing)
Molecularpourous membrane tubing is used for desalting, protein and DNA isolation
Dialysis (using dialysis tubing)
Molecularpourous membrane tubing is used for desalting, protein and DNA isolation / purification. It comes in several diameters and pore sizes (for molecular weight conversions for nucleic acids see general reference ). The brand used in M. Soares
Preparation of Dialysis tubing
1)Boil the tubing on a sitr plate (preferably in the hood)in a 4L volume of 2% (w/v)sodium bicarbonate and 1mM EDTA pH 8.0 2)Rise the tubing in distilled water thoroughly 3)Boil for 10 minutes in 1mM EDTA (pH 8.0) 4)Allow tubing to cool
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